We use essential cookies to operate our site. With your consent, we may also use non-essential cookies to improve your user experience and to analyze website traffic. Click "Accept" to accept cookies and go directly to the site, click "Reject" to reject all but cookies strictly necessary for the functioning of this site. You can reset your cookie settings at any time by visiting your Bioz "my account" page and selecting the "reset cookie preferences" link.

Accept Reject

Overview
Images
Article Snippets
home > search results > product details
star_border
     Loading Product Details ...      Welcome to Your Next Discovery   
91 / 100
Bioz Stars

ATCC  
Open an account
already a user? Sign in
are you a supplier? Contact us
Product Images (4) - All
All
Activation Assay
Enzyme-linked Immunosorbent Assay
Expressing
Flow Cytometry
In Vitro
In Vivo
Infection
Injection
Produced
Staining
Tumor Implantation
Western Blot
GITRL-armed Delta-24-RGD oncolytic adenovirus prolongs survival and induces anti-glioma immune memory 
star_border
Generation of Delta-24-GREAT. ( A ) Schematic representation of Delta-24-GREAT genomic structure. pCMV , cytomegalovirus promoter; BGH pA , bovine growth factor polyadenylation signal; ITR , inverted terminal repeat. ( B and D ) Expression in vitro of the GITR ligand (GITRL) on the surface of Delta-24-GREAT-infected murine glioma cells <t>GL261-5</t> (100 MOIs) (B) and human brain tumor stem cells GSC17 (25 MOIs) ( D ) assessed by FACS, 48 hours after infection. Mock-infected cells stained with IgG isotype as primary antibody, and UV-inactivated Delta-24-GREAT-infected cells were used as control. Non-viable cells were excluded from the analysis using ethidium homodimer. Data are shown as mean ± SD of three independent experiments. α, antibody. (C and E ) Expression of viral proteins, E1A and fiber, and murine GITRL, assessed by western blotting, in Delta-24-GREAT-infected murine glioma GL261-5 cells ( C ) and human glioma U-87MG cells (E) 48 hours after infection at the indicated MOIs. Mock, Delta-24-RGD, and UV-inactivated Delta-24-GREAT were used as controls at the highest MOI. GAPDH expression was used as loading control.
GITRL-armed Delta-24-RGD oncolytic adenovirus prolongs survival and induces anti-glioma immune memory 
star_border
In vivo effect of Delta-24-GREAT. ( A ) Schema of the preclinical study. <t>GL261-5</t> cells (5 × 10 4 cells/5 µL) were implanted intracranially (ic) in the right caudate nucleus of C57BL/6 mice using a guide-screw system. Delta-24-GREAT or Delta-24-RGD (5 × 10 7 pfu; 5 µL) was intratumorally (it) injected on day 6, 8, and 10 after tumor cell implantation. PBS was used as control. ( B ) Survival plots of experiment described in (A) showed prolonged survival with long-term survivors (>100 days) of Delta-24-GREAT-treated mice. ( n = 10, Delta-24-RGD and Delta-24-GREAT; n = 9, PBS). P = .002 (log-rank test; Delta-24-GREAT vs Delta-24-RGD). ( C ) Histopathological examination of hematoxylin and eosin stained sections of tumors of mice that showed signs of disease, and displayed high cellularity. Delta-24-GREAT-treated mice showed increased areas of necrosis. Scale bar = 100 μm. ( D ) Expression of mGITRL was analyzed by FACS 15 days after tumor implantation, and 5 days after the last viral dose. Data are represented as percentage of positive cells (3 brains were pooled for analysis). ( E ) Expression of mGITR in CD4+ and CD8+ T cells was analyzed by FACS 15 days after tumor implantation. Data are represented as mean ± SD, relative to PBS values (equal to 1) ( n = 3). * P < .05, Student’s t -test, double sided.
GITRL-armed Delta-24-RGD oncolytic adenovirus prolongs survival and induces anti-glioma immune memory 
star_border
Immune activation upon Delta-24-GREAT treatment. ( A ) Schematic representation of the time point analysis to assess immunological studies. ( B ) Frequency of infiltrating lymphocytes CD45 + CD3 + , CD45 + CD3 + CD4 + (helper T cells), and CD45 + CD3 + CD8 + (cytotoxic T cells) was analyzed by FACS on day 15 after cell implantation. Data are represented as mean ± SE, relative to PBS (equal to 1). n = 3 for CD45 + CD3 + ; n = 6 for CD45 + CD3 + CD4 + and CD45 + CD3 + CD8 + . * P < .05; *** P < .001, ns = not significant (Student’s t -test, double sided). ( C and D ) Expression of Th1-associated cytokines produced by activated splenocytes from Delta-24-GREAT-treated mice. IFNγ ( C ) and IL-2 ( D ) levels were quantified by ELISA in supernatant of splenocytes obtained from each treated group of <t>GL261-intracranial</t> bearing mice, cocultured for 48 hours with the indicated target cells. Data are represented as mean ± SD. ns = not significant; * P < .05; ** P < .01; *** P < .001 (Student’s t -test, double sided).
GITRL-armed Delta-24-RGD oncolytic adenovirus prolongs survival and induces anti-glioma immune memory Neuro-oncology Advances, 2022 Dec 14
"Generation of Delta-24-GREAT. ( A ) Schematic representation of Delta-24-GREAT genomic structure. pCMV , cytomegalovirus promoter; BGH pA , bovine growth factor polyadenylation signal; ITR , inverted terminal repeat. ( B and D ) Expression in vitro of the GITR ligand (GITRL) on the surface of Delta-24-GREAT-infected murine glioma cells <t>GL261-5</t> (100 MOIs) (B) and human brain tumor stem cells GSC17 (25 MOIs) ( D ) assessed by FACS, 48 hours after infection. Mock-infected cells stained with IgG isotype as primary antibody, and UV-inactivated Delta-24-GREAT-infected cells were used as control. Non-viable cells were excluded from the analysis using ethidium homodimer. Data are shown as mean ± SD of three independent experiments. α, antibody. (C and E ) Expression of viral proteins, E1A and fiber, and murine GITRL, assessed by western blotting, in Delta-24-GREAT-infected murine glioma GL261-5 cells ( C ) and human glioma U-87MG cells (E) 48 hours after infection at the indicated MOIs. Mock, Delta-24-RGD, and UV-inactivated Delta-24-GREAT were used as controls at the highest MOI. GAPDH expression was used as loading control. "
Protocol Conditions
Article Snippets for All Techniques

ATCC logo

ATCC is the premier global biological materials resource and standards organization whose mission focuses on the acquisition, authentication, production, preservation, development, and distribution of standard reference microorganisms, cell lines, and other materials. While maintaining traditional collection materials, ATCC develops high quality products, standards, and services to support scientific research and breakthroughs that improve the health of global populations.